BioChek has introduced a Pneumovirus (aMPV) indirect ELISA test kit to detect antibodies against aMPV subtypes A, B and C. The test kits can be used to assay both turkey and chicken sera.
With the advent of aMPV infection in broilers, commercial laying flocks, and growing turkeys in North America, there is a need to identify the presence of antibodies to differentiate among respiratory viral infections.
Applying the BioChek ART (aMPV) ELISA is a convenient and effective technique for monitoring flocks, although as yet no approved vaccine is currently available in the U.S. as in Europe.
To obtain registration for the test kit, BioChek demonstrated high specificity with negative results on 80 sera derived from SPF chickens. Negative titers were obtained when assaying sera with diagnostic levels of antibody against a range of avian pathogens, including adenovirus, IBV, IBD, AI, ILT, NDV, and EDS denoting high specificity. Assayed ELISA Titers ranged from non-detectable to 800 units, with a 1,600-unit cutoff denoting infection.
With respect to assays on field samples, sensitivity was confirmed by quantitative antibody detection with either field strains of aMPV, yielding ELISA values over 15,000 in chickens vaccinated with Nemovac™ (Boehringer-Ingelheim) PL21 type B. Applying the BioChek system, ELISA values in the range of 6,600 were measured 48 days after vaccination.
The BioChek ART (aMPV) ELISA test kit is a highly effective addition to the range of antibody detection kits supplied by the Company for diagnostic laboratories. The availability of a sensitive but specific aMPV antibody assay will allow poultry health professionals to confirm exposure of chicken and turkey flocks to the pathogen. Structured programs of assay can define the epidemiology of infection and lead to appropriate preventive strategies before and after vaccines become available.
For technical specifications for the aMPV ELISA kit activate https://www.biochek.com/poultry-elisa/avian-rhinotracheitis-antibody-test-kit/
Additional information on the BioChek range of diagnostic kits click on to the Company logo on the right side of the welcome page