The Ohio Animal Disease Diagnostic Laboratory is now offering two assay procedures to diagnose avian metapneumovirus infection (aMPV).
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A real time-PCR assay has been developed to detect aMPV nucleic acid from samples including tracheal and choanal swabs. Dacron swabs should be used for sampling and specimens should be transported in a compatible medium. RT-PCR assays are conducted on Monday through Friday with a charge of $36 per test. The procedure will detect and differentiate among all three subgroups of aMPV.
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An indirect ELISA procedure will detect antibodies to aMPV. The procedure is group specific and therefore will not differentiate between subgroups A, B and C nor distinguish between vaccinated and infected flocks. Samples are assayed on Tuesdays and Thursdays at a cost of $5 per sample.